<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">periodontology</journal-id><journal-title-group><journal-title xml:lang="ru">Пародонтология</journal-title><trans-title-group xml:lang="en"><trans-title>Parodontologiya</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1683-3759</issn><issn pub-type="epub">1726-7269</issn><publisher><publisher-name>Russian Periodontal Association (RPA)</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.33925/1683-3759-2021-26-2-170-174</article-id><article-id custom-type="elpub" pub-id-type="custom">periodontology-490</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>ИССЛЕДОВАНИЕ</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>RESEARCH</subject></subj-group></article-categories><title-group><article-title>Optimization of periodontal disease diagnosis by the results of clinical laboratory tests</article-title><trans-title-group xml:lang="en"><trans-title>Optimization of periodontal disease diagnosis by the results of clinical laboratory tests</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3065-6831</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Al-Qufaish</surname><given-names>M.A.M.</given-names></name><name name-style="western" xml:lang="en"><surname>Al-Qufaish</surname><given-names>M.A.M.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Аль Кофиш Мохаммед Али Мохаммед, кандидат медицинских наук, заместитель декана стоматологического факультета</p><p>Аден, Йемен</p></bio><bio xml:lang="en"><p>Mohammed A.M. Al-Qufaish, MD, PhD (Medicine), deputy dean of the dental school of University of Aden </p><p>Aden, Yemen</p></bio><email xlink:type="simple">alqufaesh@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1781-0291</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Usmanova</surname><given-names>I. N.</given-names></name><name name-style="western" xml:lang="en"><surname>Usmanova</surname><given-names>I. N.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Усманова Ирина Николаевна, доктор медицинских наук, профессор кафедры терапевтической стоматологии</p><p>Уфа</p></bio><bio xml:lang="en"><p>Irina N. Usmanova, Dr. Sci. (Med.), Рrofessor of the Department of therapeutic dentistry </p><p>Ufa</p></bio><email xlink:type="simple">irinausma@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5473-2034</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Tuigunov</surname><given-names>M. М.</given-names></name><name name-style="western" xml:lang="en"><surname>Tuigunov</surname><given-names>M. М.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Туйгунов Марсель Маратович, доктор медицинских наук, профессор, завeдующий кафедрой микробиологии и вирусологии</p><p>Уфа</p></bio><bio xml:lang="en"><p>Marcel M. Tuigunov, Dr. Sci. (Med.), Рrofessor, Нead of the Department of Microbiology and Virology </p><p>Ufa</p></bio><email xlink:type="simple">tuygunov@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1001-9587</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Khusnarizanova</surname><given-names>R. F.</given-names></name><name name-style="western" xml:lang="en"><surname>Khusnarizanova</surname><given-names>R. F.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Хуснаризанова Рауза Фазыловна, кандидат медицинских наук, доцент кафедры микробиологии и вирусологии</p><p>Уфа</p></bio><bio xml:lang="en"><p>Rausa F. Khusnarizanova, Ph.D. (Med.), Аssociate Professor of the Department of Microbiology and Virology </p><p>Ufa</p></bio><email xlink:type="simple">roza.khusna@mail.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5765-4273</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Gumerova</surname><given-names>M. I.</given-names></name><name name-style="western" xml:lang="en"><surname>Gumerova</surname><given-names>M. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Гумерова Марьям Ильдаровна, кандидат медицинских наук, доцент кафедры терапевтической стоматологии; врач стоматолог-терапевт стоматологической клиники «Мой стоматолог»</p><p>Уфа</p></bio><bio xml:lang="en"><p>Maryam I. Gumerova, MD, PhD, Аssociate Professor; Operative dentist of dental clinic</p><p>Ufa</p></bio><email xlink:type="simple">maguil@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9673-1654</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Shangareeva</surname><given-names>A. I.</given-names></name><name name-style="western" xml:lang="en"><surname>Shangareeva</surname><given-names>A. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Шангареева Алия Ирековна, ассистент кафедры терапевтической стоматологии; Врач-стоматолог-пародонтолог клиники </p><p>Уфа</p></bio><bio xml:lang="en"><p>Aliya I. Shangareeva, PhD, assistant professor Department of therapeutic dentistry; Dentist-periodontist </p><p>Ufa</p></bio><email xlink:type="simple">180780_lena@mail.ru</email><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>University of Aden, School of Dentistry</institution><country>Йемен</country></aff><aff xml:lang="en"><institution>University of Aden, School of Dentistry</institution><country>Yemen</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Bashkir State Medical University</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Bashkir State Medical University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Bashkir State Medical University;&#13;
«My dentist» Dental clinic</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Bashkir State Medical University;&#13;
«My dentist» Dental clinic</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Bashkir State Medical University;&#13;
«Kremlin-Denta» Dental clinic</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Bashkir State Medical University;&#13;
«Kremlin-Denta» Dental clinic</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>30</day><month>06</month><year>2021</year></pub-date><volume>26</volume><issue>2</issue><fpage>170</fpage><lpage>174</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Al-Qufaish M., Usmanova I.N., Tuigunov M.М., Khusnarizanova R.F., Gumerova M.I., Shangareeva A.I., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Al-Qufaish M., Usmanova I.N., Tuigunov M.М., Khusnarizanova R.F., Gumerova M.I., Shangareeva A.I.</copyright-holder><copyright-holder xml:lang="en">Al-Qufaish M., Usmanova I.N., Tuigunov M.М., Khusnarizanova R.F., Gumerova M.I., Shangareeva A.I.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.parodont.ru/jour/article/view/490">https://www.parodont.ru/jour/article/view/490</self-uri><abstract><sec><title>Relevance</title><p>Relevance. The authors have established that the microbiological and local risk factors prevail in changing the clinical condition of the periodontium. Aim – сlinical and diagnosis argumentation of the gingival tissue condition according to the criteria of the New International Classification of Periodontal and Peri-implant Diseases and Conditions and Proceedings of the 2017 World Workshop jointly held together by the American Association of Periodontology (AAP) and the European Federation of Periodontology (EFP).</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Clinical and laboratory assessment of 105 young patients was conducted. Three patient groups were formed according to the detected risk factor and according to the data of the New International Classification of Periodontal and Peri-implant Diseases and Conditions. The main group consisted of 70 (66.6%) patients with diagnosed chronic plaque-induced gingivitis (33.3%) and initial periodontitis (33.3%) (mild chronic periodontitis). The control group comprised 35 patients with clinically healthy gingiva on an intact periodontium (71.1%) and reduced periodontium (22.9%). Periodontal pathogens as a risk factor were assessed by PCR using DNA-express commercial sets (Liteh, LLC, scientific manufacturing company, Russia). Cytology of the gingival crevicular fluid impression smears stained by Romanovsky-Giemsa method was performed.</p></sec><sec><title>Results</title><p>Results. Changes in the hygiene and periodontal indices were revealed on full dental examination. PCR detected low or critical number of periodontal pathogens in the studied samples. Neutrophilic leukocytes, histiocytes and epithelial cells were present in the impression smears, polymorphonyclear neutrophils significantly increased and macrophages, histiocytes, epithelial cells appeared; macrophages decreased.</p></sec><sec><title>Conclusion</title><p>Conclusion. Full dental examination and laboratory tests revealed the following clinical conditions: clinically healthy gingiva on an intact periodontium, clinically healthy gingiva on a reduced periodontium, plaque-induced gingivitis, stage I periodontitis – initial periodontitis, which corresponded to the New International Classification of Periodontal and Peri-Implant Diseases and Conditions and Proceedings of the 2017 World Workshop held by the American Academy of Periodontology (AAP) and the European Federation of Periodontology (EFP).</p></sec></abstract><trans-abstract xml:lang="en"><sec><title>Relevance</title><p>Relevance. The authors have established that the microbiological and local risk factors prevail in changing the clinical condition of the periodontium. Aim – сlinical and diagnosis argumentation of the gingival tissue condition according to the criteria of the New International Classification of Periodontal and Peri-implant Diseases and Conditions and Proceedings of the 2017 World Workshop jointly held together by the American Association of Periodontology (AAP) and the European Federation of Periodontology (EFP).</p></sec><sec><title>Materials and methods</title><p>Materials and methods. Clinical and laboratory assessment of 105 young patients was conducted. Three patient groups were formed according to the detected risk factor and according to the data of the New International Classification of Periodontal and Peri-implant Diseases and Conditions. The main group consisted of 70 (66.6%) patients with diagnosed chronic plaque-induced gingivitis (33.3%) and initial periodontitis (33.3%) (mild chronic periodontitis). The control group comprised 35 patients with clinically healthy gingiva on an intact periodontium (71.1%) and reduced periodontium (22.9%). Periodontal pathogens as a risk factor were assessed by PCR using DNA-express commercial sets (Liteh, LLC, scientific manufacturing company, Russia). Cytology of the gingival crevicular fluid impression smears stained by Romanovsky-Giemsa method was performed.</p></sec><sec><title>Results</title><p>Results. Changes in the hygiene and periodontal indices were revealed on full dental examination. PCR detected low or critical number of periodontal pathogens in the studied samples. Neutrophilic leukocytes, histiocytes and epithelial cells were present in the impression smears, polymorphonyclear neutrophils significantly increased and macrophages, histiocytes, epithelial cells appeared; macrophages decreased.</p></sec><sec><title>Conclusion</title><p>Conclusion. Full dental examination and laboratory tests revealed the following clinical conditions: clinically healthy gingiva on an intact periodontium, clinically healthy gingiva on a reduced periodontium, plaque-induced gingivitis, stage I periodontitis – initial periodontitis, which corresponded to the New International Classification of Periodontal and Peri-Implant Diseases and Conditions and Proceedings of the 2017 World Workshop held by the American Academy of Periodontology (AAP) and the European Federation of Periodontology (EFP).</p></sec></trans-abstract><kwd-group xml:lang="ru"><kwd>clinically healthy gingiva</kwd><kwd>intact periodontium</kwd><kwd>gingivitis</kwd><kwd>initial periodontitis</kwd><kwd>microbial biofilm</kwd><kwd>periodontal pathogens</kwd></kwd-group><kwd-group xml:lang="en"><kwd>clinically healthy gingiva</kwd><kwd>intact periodontium</kwd><kwd>gingivitis</kwd><kwd>initial periodontitis</kwd><kwd>microbial biofilm</kwd><kwd>periodontal pathogens</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Гуревич КГ, Теблоева ЛМ. Этиология и патогенез пародонтита. Современный взгляд. Пародонтология: национальное руководство. 2-е издание. Москва: ГЭОТАР-Медиа. 2018:55-63. Режим доступа: https://www.rosmedlib.ru/doc/ISBN9785970443651-0007.html.</mixed-citation><mixed-citation xml:lang="en">Gurevich KG., Tebloeva LM. Etiology and pathogenesis of periodontitis. Modern view. Periodontology: a national guide. 2nd edition. Moscow: GEOTAR-Media. 2018:55-63. (In Russ.). Available from: https://www.rosmedlib.ru/doc/ISBN9785970443651-0007.html.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Дмитриева ЛА, Грудянов АИ, Ревазова ЗЭ. Клинические проявления и лечение заболеваний пародонта. Пародонтология: национальное руководство. 2-е издание. Москва: ГЭОТАР-Медиа. 2018:245-288. Режим доступа: https://www.rosmedlib.ru/doc/ISBN9785970443651-0015.html.</mixed-citation><mixed-citation xml:lang="en">Dmitrieva LA, Grudyanov AI, Revazova ZE. Clinical manifestations and treatment of periodontal diseases. Editors. Periodontology: a national guide. 2nd edition. Moscow: GEOTAR-Media. 2018:245-288. (In Russ.). Available from: https://www.rosmedlib.ru/doc/ISBN9785970443651-0015.html.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Аль-Кофиш МАМ. Оптимизация ранней диагностики, профилактики и лечения воспалительных заболеваний пародонта у лиц молодого возраста. Диссертация на соискание ученой степени кандидата медицинских наук. Башкирский государственный медицинский университет. Уфа. 2019:151. Режим доступа: https://elibrary.ru/item.asp?id=41521324.</mixed-citation><mixed-citation xml:lang="en">Al-Qufaish MAM. Optimization of early diagnosis, prevention and treatment of inflammatory periodontal diseases in young people. Dissertation for the degree of Candidate of Medical Sciences. Bashkir State Medical University. Ufa. 2019:151. (In Russ.). Available from: https://elibrary.ru/item.asp?id=41521324.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Орехова ЛЮ, Кудрявцева ТВ, Чеминава НР, Тачалов ВВ, Лобода ЕС. Проблемы стоматологического здоровья у лиц молодого возраста (обзор литературы). Пародонтология. 2014;2(71):3-5. Режим доступа: https://elibrary.ru/item.asp?id=22135425.</mixed-citation><mixed-citation xml:lang="en">Orekhova LYu, Kudryavtseva TV, Cheminava NR, Tachalov VV, Loboda ES. Oral health problems for young adults (review). Parodontologyia. 2014; 2(71):3-5 (In Russ). Available from: https://elibrary.ru/item.asp?id=22135425.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Könönen E, Gursoy M, Gursoy UK. Periodontitis: A Multifaceted Disease of Tooth-Supporting Tissues. Journal of Clinical Medicine. 2019;8(8):1135. https://doi.org/10.3390/jcm8081135.</mixed-citation><mixed-citation xml:lang="en">Könönen E, Gursoy M, Gursoy UK. Periodontitis: A Multifaceted Disease of Tooth-Supporting Tissues. Journal of Clinical Medicine. 2019;8(8):1135. https://doi.org/10.3390/jcm8081135.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Цепов ЛМ, Николаев АИ, Нестерова ММ, Цепова ЕЛ. Патогенетические особенности формирования хронической воспалительной патологии пародонта (обзор). Вестник Смоленской государственной медицинской академии. 2018;(17)3:206-214. Режим доступа: https://elibrary.ru/item.asp?id=35722174.</mixed-citation><mixed-citation xml:lang="en">Tsepov LM, Nikolaev AI, Nesterova MM, Tsepova EL. Pathogenetic features of chronic periodontal pathology development (review). Vestnik of the smolensk state medical academy. 2018; (17)3: 198-205 (In Russ.). Available from: https://elibrary.ru/item.asp?id=35722174.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Цепов ЛМ, Николаев АИ, Наконечный ДА, Нестерова ММ. Микробные биопленки и хронические воспалительные заболевания пародонта (обзор литературы). Пародонтология. 2015;3 (76):3-6. Режим доступа: https://elibrary.ru/item.asp?id=24365624.</mixed-citation><mixed-citation xml:lang="en">Tsepov LM, Nikolaev AI, Nakonechnyi DA, Nesterova MM. Microbial biofilms and chronic inflammatory periodontal diseases (a review of the literature). Parodontologyia. 2015;3 (76):3-6. (In Russ.). Available from: https://elibrary.ru/item.asp?id=24365624.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Лукичев ММ, Ермолаева ЛА. Современные представления о роли микрофлоры в патогенезе заболеваний пародонта. Институт стоматологии. 2018;1(78):92-94. Режим доступа: https://www.elibrary.ru/item.asp?id=34964799.</mixed-citation><mixed-citation xml:lang="en">Lukichev MM, Ermolaeva LA. Modern ideas about therole of microflora in the pathogenesis of periodontal diseases. Institute of Dentistry.2018;1(78):92-94. (In Russ.). Available from: https://www.elibrary.ru/item.asp?id=34964799.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Ланг НП, Бартольд МП. Здоровый пародонт. Пародонтология. 2019;2(24):187-192. Режим доступа: https://elibrary.ru/item.asp?id=39195270.</mixed-citation><mixed-citation xml:lang="en">Lang NP, Bartold MP. Periodontal health. Parodontologyia. 2019;2(24):187-192. (In Russ). Available from: https://elibrary.ru/item.asp?id=39195270.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
