The analysis of the microbial composition of the biotopes of oral cavity in young patients depending on the dental status

Abstract

The paper presents the analysis of the microbial composition in different biotopes of oral cavity in young patients depending on the dental status. A comprehensive clinical examination of 105 young adults (55 men and 50 women) aged 20 to 35 years. Microbiological examination included the study of samples of different biotopes of oral cavity with use of selective and non-selective media, as well as the test system for the qualitative detection of the presence of the pathogen and its localization by PCR. Using polymerase chain reaction samples samples of plaque, and gingival fluid content of the periodontal pocket to determine the qualitative composition of periodontopathogenic microorganisms and endodеntalis Porphyromonas gingivalis, Tannerella forsythensis, Treponema denticola, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum at persons of young age chronic catarrhal gingivitis and periodontitis mild severity, as well as in patients with clinically intact periodontium. Depending on the clinical condition of periodontal tissues in the total microflora of different biotopes of oral cavity significantly increases the incidence of detection of periodontopathogenic micro organisms such as Porphyromonas gingivalis, Fusobacterium nucleatum and Aggregatibacter actinomycetemcomitans, on the background of identifying the pathogenic lactic acid bacteria and yeast-like fungi of the genus Candida. Obtained in the course of the study, data of prognostic importance not only for the diagnosis of inflammatory periodontal diseases, but also for the development of effective therapeutic and preventive measures in young depending on the clinical condition of periodontal tissues.