Pilot study of antimicrobic and anti-biofilm activity of a combination of Ciprofloxacin and Tinidazolum in vitro

Relevance. Development of a chronic periodontal disease is associated with forming microbic a biofilms therefore the research of anti-biofilm activity of drugs allows to expand possibilities of their effective use for treatment.
Purpose. Determination of activity of antimicrobic components (ciprofloxacin, tinidazolum) the combined drug (Сiphran ST) on planktonic and biofilm forms of pathogens in comparison with amoxicillin/clavulanate.
Materials and methods. The pilot study of influence on planktonic forms of pathogens is executed with use of a technique of the automated cultivation of in vitro of strains of S. aureus (MRSA) and P. intermedia in a biocultivator of «Revers-Spinner RTS-1» (BioSan, Latvia). Impact on a microbic biofilm was estimated on ternary model (S. sanguis, F. nucleatum, P. intermedia) with use of the scanning electronic microscopy.
Results. At impact on a strain of MRSA MIC of ciprofloxacin/tinidazolum made 12.5/500 and 25/250 mkg/ml while for the amoxicillin/klavulanat the inhibiting action was fixed only in very high concentration – 500 mkg/ml; at impact on MIC P. intermedia of ciprofloxacin/tinidazolum made 25/250 mkg/ml that did not concede to effect of amoxicillin/clavulanate. When comparing impact of the studied medicines on a three-component biofilm the concentration coming to MIC established destruction of a mantle of a biofilm and partial damage of microbial cells under the influence of ciprofloxacin/tinidazolum (according to the scanning electronic microscopy) while amoxicillin/clavulanate did not render such effect.
Conclusion. Results showed advantages of components (ciprofloxacin, tinidazolum) the combined drug (Ciphran ST) at impact on strains resistant to amoxicillin and on biofilms as choice medicine for complex treatment of patients with a chronic periodontal disease.

chronic periodontal disease, planktonic forms, biofilm, anti-biofilm activity, ciprofloxacin, tinidazolum, amoxicillin

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