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Experimental study of the Ti-implant surfaces structured by the ytterbium-doped pulsed fiber laser

https://doi.org/10.33925/1683-3759-2021-26-2-88-95

Abstract

Relevance. Currently, there are several basic techniques for the dental implant surface structuring. Laser treatment is an extremely promising technique for the surface structuring. This technology allows creating regular implant surface without using chemicals and in just one technological step. The purpose was to present study aimed to compare and evaluate in vivo the stability and osseointegration of dental implants with 2 different surfaces structured by ytterbium-doped pulsed fiber laser operating at 1064 nm.

Materials and methods. 60 dental implants were placed in the study. 2 types of dental implant surfaces, namely holes and parallel grooves, were created by the ytterbium laser operating at 1064 nm. A polished dental implant (without laser surface structuring) was also included in the experiment for comparison. The study was carried out on 15 laboratory animals (male rabbits, weight 3.5-4 kg). The implants were placed in the tibia. 4 implants with different surface types but of the same diameter and length were placed in each rabbit.

Results. Laboratory animals were sacrificed 1.5 and 3 months after the surgery. The stability of the implants was assessed by RFA (Resonance Frequency Analysis), based on the registration of resonance electromagnetic oscillations of the implant and the surrounding bone when they are exposed to the electromagnetic field (Osstell ISQ). Also, nondecalcified bone blocks were histologically examined using a confocal laser scanning microscope (Carl Zeiss LSM 780) and histomorphometry was performed (BIC-index: Bone-to-implant contact). Bone blocks were prepared according to a special technique: they were soaked and embedded into the plastic and synthetic resin. The obtained blocks were cut into sections, 40-50 µm thick, and stained with toluidine blue.

Conclusion. Laser surface structuring of the dental titanium implants is a promising technique. 59 in 60 (98.3%) implants were osseointegrated, there were no signs of inflammation in the bone tissue. The present results allow further studying of dental implants with various surface designs, structured by ytterbium laser.

About the Authors

A. I. Yaremenko
Pavlov First Saint-Petersburg State Medical University
Russian Federation

Andrei I. Yaremenko, Dr. Sci. (Med.), Professor, Head of the Department of Oral and Maxillofacial Surgery, president of Russian Dental Association, president of Saint Petersburg Dental Association, Saint Petersburg Chief Dentist

Saint Petersburg



E. A. Zernitskaya
Pavlov First Saint-Petersburg State Medical University
Russian Federation

Ekaterina A. Zernitskaya, MD, PhD-student of the Department of Oral and Maxillofacial Surgery

Saint Petersburg



P. A. Zotov
Pavlov First Saint-Petersburg State Medical University
Russian Federation

Pavel A. Zotov, MD, resident of the Department of Oral and Maxillofacial Surgery

Saint Petersburg



Yu. Yu. Karlagina
ITMO University
Russian Federation

Yulia Yu. Karlagina, engineer, International Scientific Laboratory «Laser Micro-and Nanotechnologies», PhD-student of school of laser photonics and optical electronics

Saint Petersburg



E. E. Egorova
ITMO University
Russian Federation

Elena E. Egorova, engineer, International Scientific Laboratory «Laser Micro- and Nanotechnologies», School of laser photonics and optical electronics

Saint Petersburg



G. V. Odintsova
ITMO University
Russian Federation

Galina V. Odintsova, PhD (Technical Sciences), Scientific Researcher, International Scientific Laboratory «Laser Micro- and Nanotechnologies», School of Laser Photonics and Optical Electronics 

Saint-Petersburg



References

1. Albrektsson T, Wennerberg A. On osseointegration in relation to implant surfaces. Clinical Implant Dentistry and Related Research. 2019;21:4-7. https://doi.org/10.1111/cid.12742.

2. Velasco-Ortega E, Alfonso-Rodríguez CA, MonsalveGuil L, EspañaLópez A, Jiménez-Guerra A, Garzón I, Alaminos M, Gil FJ. Relevant aspects in the surface properties in titanium dental implants for the cellular viability. Materials Science and Engineering. 2016;64:1-10. https://doi.org/10.1016/j. msec.2016.03.049.

3. Jemat A, Ghazali MJ, Razali M, Otsuka Y. Surface Modifications and Their Effects on Titanium. Dental Implants. Biomed Research International. 2015;3:1-11. https://doi.org/10.1155/2015/791725.

4. Milleret V, Lienemann PS, Gasser A, Bauer S, Ehrbar M, Wennerberg A. Rational design and in vitro characterization of novel dental implant and abutment surfaces for balancing clinical and biological needs. Clinical Implant Dentistry and Related Research. 2019;21:15-24. https://doi.org/10.1111/cid.12736.

5. Lee JT, Cho SA. Biomechanical evaluation of laseretched Ti implant surfaces vs. chemically modified SLA Ti implant surfaces: Removal torque and resonance frequency analysis in rabbit tibias. Journal of the Mechanical Behavior of Biomedical Materials. 2016;61:299-307. https://doi.org/10.1016/j.jmbbm.2016.03.034.

6. Simitzi C, Ranella A, Stratakis E. Controlling the morphology and outgrowth of nerve and neuroglial cells: The effect of surface topography. Acta biomaterialia. 2017;51:21-52. https://doi.org/10.1016/j.actbio.2017.01.023

7. Volkov AV. Histomorphological researches of relationship of a bone with dental implant. Biomedicina. 2012;4:96-100. (In Russ.). Available from: https://cyberleninka.ru/article/n/gistomorfologicheskieissledovaniya-vzaimootnosheniy-kostnoy-tkani-s-dentalnym-implantatom.

8. Volkov AV, Smbatyan BS, Nazaryan DN, Muraev AA. A novel morphometric nomenclature to evaluate osseointegration of intraosseous implants. Sovremennye tehnologii v medicine. 2018;10(3):7-13. (In Russ.). https://doi.org/10.17691/stm2018.10.3.1.

9. Calvo-Guirado JL., Perez-Albacete Martínez C, Negri B, Delgado-Ruíz R. Mechanical, histological and histomorphometric evaluation of modified by femtosecond laser zirconia implants versus titanium implants. An experimental study in dogs at three months. Journal of Osseointegratoin. 2013;5(2):19-26. https://doi.org/10.23805/jo.2013.05.02.01.

10. Veiko VP, Karlagina YuYu, Romanov VV, Yatsuk RM, Egorova EE, Zernitskaya ЕА, Yaremenko АI, Chernenko GN, Gorny SG, Odintsova GV. Laser Technology for Structuring the Surface of Dental Titanium Implants. Photonics. 2020;5(14):462-472 (In Russ.). https://doi.org/10.22184/1993-7296.FRos.2020.14.5.462.472.


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For citations:


Yaremenko AI, Zernitskaya EA, Zotov PA, Karlagina YY, Egorova EE, Odintsova GV. Experimental study of the Ti-implant surfaces structured by the ytterbium-doped pulsed fiber laser. Parodontologiya. 2021;26(2):88-95. (In Russ.) https://doi.org/10.33925/1683-3759-2021-26-2-88-95

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ISSN 1683-3759 (Print)
ISSN 1726-7269 (Online)