Customized in-vivo tissue engineering for bone grafting
https://doi.org/10.33925/1683-3759-2023-28-1-49-54
Abstract
Relevance. Jaw bone volume restoration during dental implantation and reconstructive oral surgery is a relevant problem in modern dentistry. In recent years, the needs of daily dental practice determined the search for new osteoplastic materials with desired properties, including cellular technologies, to stimulate bone regeneration and accelerate bone repair processes.
Materials and methods. The study used third molar area gingival specimens to create tissue-engineered constructs for bone matrix colonization, subject to in vitro expansion. Octacalcium-phosphate-based materials (OCP), used as the carrier matrix, were characterized by a larger particle surface area for a more developed microrelief, a bioresorption rate, and a hydrophilic surface. The finished tissue-engineered construct, consisting of multipotent mesenchymal stromal cells colonized on the matrix, was implanted into an artificially created tibial defect in 8 Chinchilla male rabbits. Animal experiments were conducted according to ethical standards. Rabbits were sacrificed on days 8 and 12 for histological testing.
Results. In the early follow-up period (8 weeks), there were areas of mature bone with incorporated osteoblasts. Besides, there were areas of primary bone with adhesion lines. Later (12 weeks), such granules fully integrated into the diaphysis cortical part. The results showed the preservation of the low-mineralized bone girdle, osteoid - a bone substance formation precursor, between the octacalcium phosphate granule and the bone.
Conclusion. The results of the experimental study allow us to conclude that the customized tissue-engineered construct developed by us contributes to bone grafting.
About the Author
I. I. Tarba
A. I. Yevdokimov Moscow State University of Medicine and Dentistry
Russian Federation
Russian Federation
Ilona I. Tarba, Assistant of the Department of Oral Surgery
Moscow
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Review
For citations:
Tarba II. Customized in-vivo tissue engineering for bone grafting. Parodontologiya. 2023;28(1):49-54. (In Russ.) https://doi.org/10.33925/1683-3759-2023-28-1-49-54
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