Histological and immunohistochemical analysis of autografts and xenografts synergy in rectifying attached keratinized gingiva deficiency

Relevance. The complex, multi-stage surgical procedure aimed for replacing missing teeth often results in a deficiency of attached keratinized gingiva (AKG) in the intervened area. Despite numerous methods available for soft tissue volume and esthetics reconstruction, only a few demonstrate considerable long-term stability post-surgery. The quest to address this intricate issue has led to the combined utilization of autografts and xenografts Consequently, there is a need to evaluate the tissue structure obtained through this synergy via histological and immunohistochemical examination of the oral mucosa in the reconstructed region.

Materials and methods. This study involved analyzing 87 biopsies of oral mucosa obtained from 57 patients aged 28 to 59 years who had undergone previous soft tissue volume and esthetics reconstruction in the AKG zone. Histological and immunohistochemical examinations were conducted on these biopsies, collected six weeks post soft tissue operation. A section of oral mucosa from the hard palate served as the reference sample.

Results. The epithelial structure in the examined groups exhibited a well-defined basal layer with a continuous structure comprising two layers of cells without notable atypia, indicating a high cell proliferation capability. Immunohistochemical analysis targeting protein molecules revealed an absence of inflammation. The expression results of the Ki-67 antigen suggested the preservation of cell proliferation capacity and, consequently, the maintenance of tissue regenerative potential at a high level during the remote postoperative period, up to six weeks.

Conclusion. This research showcases the efficacy of employing a combination of autografts and xenografts, a conclusion drawn from comprehensive histological and immunohistochemical analyses. Consequently, this approach presents a promising and innovative alternative to conventional methods for soft tissue volume and esthetics reconstruction.

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