Features of progress of chronic periodontitis associated with biofilm-forming bacteria

Abstract

The formation of biofilms by oral bacteria is an important link in the pathogenesis of chronic periodontitis. The study of new approaches for identification and research of biofilms, the search for mechanisms of influence on the formation and destruction of biofilms, is a promising and relevant scientific and practical direction. The aim of the study was to evaluate the course of chronic periodontitis associated with biofilm-forming microorganisms and to study the stability of the biofilm formed by them to enzymes, antiseptics and antibacterial drugs. 97 patients with chronic periodontitis and 30 patients with intact periodontitis were examined. In the study, the properties of 127 clinical isolates isolated in patients with chronic periodontitis and in the control group were studied, DNA of 323 periodontopathogens was determined by real-time PCR. It is established that in patients with chronic periodontitis there are changes in the microflora of the oral cavity: the ability of bacteria to form biofilm increases and their resistance to antibiotics increases. Proteinase K and dimethylsulfoxide possessed the greatest ability to destroy the exopolymer matrix of biofilms.