The microbial landscape of the contents of periodontal pockets and root canals in patients with lesions endodonto-periodontal IV class

Abstract

Aim: to prove necessity of endodontic treatment of teeth in patients with endoperio lesions and secondary endodontic involvement. GI, electro-odonto-diagnosis, periodontal pocket depth (PD) were used to assess condition of periodontium and pulp in 70 patients of 28-60 y.o. Alveolar bone was evaluated using cone-beam computered tomography (CBCT). The main group included 50 teeth with retrograde pulpitis in severe generalized periodontitis patients. Control group consisted of 20 teeth in periodontally healthy patients with pulpitis. PCR was used for microbiological evaluation of content of periodontal pocket (PP) and of root canal (RC). Periodontal pathogen detection rate in patients of the main group was as follows: P. i. RC - 26,9% and PP - 26,9; T. f. RC - 57,7% and PP - 46,2% ; T. d. RC - 26,9% and PP - 26,9% A. a. RC - 50%, PP - 61,5%. P. g. RC - 53,5% and PP - 53,8%. Patients of the control group showed the following rate of periodontal pathogen detection: P. i. RC and gingival sulcus (GS) - 0%. T. f. RC - 8,3% and GS - 0%; T. d. RC and GS - 0%. A. a. RC - 16,7% and GS - 25%; P. g. RC - 16,7%. and GS - 16,7%. It was established that microbial composition of content of root canals surrounded by deep bone defects is identical to microflora of a periodontal pocket. Thus root canal treatment for secondary endodontic involvement is indispensable before surgery.