Relevance. Recent studies investigating the role of the microbial gaseous substances (O₂, N₂, CO₂, CH₄, NO, CO, H₂S) indicate not only in the regulation of the host's metabolic activity and the functioning of its nervous system, in particular but also their participation the pathogenesis of some diseases. However, there is scarce data in the national and international literature on the production of gas signaling molecules by the oral microbiota (Streptococcus spp. and Staphylococcus spp.) and the changes in the gas composition during the development of chronic inflammatory periodontal diseases.

Material and methods. The study included 69 people. The main group included 36 patients aged 35 to 67 years with clinically confirmed moderate chronic generalized periodontitis. The control group included 33 patients aged 27 to 55 without periodontal disease. The samples from the back of the tongue were the study material. The gas chromatography determined the production of gas signaling molecules using the Khromatek-crystal 5000.2 device. The measurement of the amount of released gases was in % (for O₂, N₂) and ppm (0.001 mg/mL) for other gas molecules (CO₂, CH₄, NO, CO, H₂S).

Results. The metabolic activity of streptococci only for the production of NO (p = 0.002) and CO (p = 0.008) appeared to have a statistically significant difference. In periodontal inflammation, there was practically no NO emission by Streptococci spp., and the concentration of CO was ten times higher than in the group of healthy individuals. The difference in the number of other signaling gas molecules was not statistically significant (p > 0.05) in healthy people and patients with chronic generalized periodontitis.

In the production of gasotransmitters among Staphylococcus spp., N₂ production (p = 0.007, increasing in the comparison group) was statistically significantly different. As in the streptococcal sampling, the amount of CO significantly increased in periodontal inflammation. Certain species of staphylococci showed a significant decrease in the production of the entire gas molecule range in the main group. At the same time, unlike Streptococcus spp., Staphylococcus spp. absorbed a much higher amount of nitric oxide in chronic periodontitis.

Conclusion. In patients with chronic generalized periodontitis and inflammation, the oral microbiota is poorly active and produces a low concentration of gasotransmitters, so they cannot participate in inflammatory process reduction, thereby contributing to the progression of the disease.

Relevance. The complex, multi-stage surgical procedure aimed for replacing missing teeth often results in a deficiency of attached keratinized gingiva (AKG) in the intervened area. Despite numerous methods available for soft tissue volume and esthetics reconstruction, only a few demonstrate considerable long-term stability post-surgery. The quest to address this intricate issue has led to the combined utilization of autografts and xenografts Consequently, there is a need to evaluate the tissue structure obtained through this synergy via histological and immunohistochemical examination of the oral mucosa in the reconstructed region.

Materials and methods. This study involved analyzing 87 biopsies of oral mucosa obtained from 57 patients aged 28 to 59 years who had undergone previous soft tissue volume and esthetics reconstruction in the AKG zone. Histological and immunohistochemical examinations were conducted on these biopsies, collected six weeks post soft tissue operation. A section of oral mucosa from the hard palate served as the reference sample.

Results. The epithelial structure in the examined groups exhibited a well-defined basal layer with a continuous structure comprising two layers of cells without notable atypia, indicating a high cell proliferation capability. Immunohistochemical analysis targeting protein molecules revealed an absence of inflammation. The expression results of the Ki-67 antigen suggested the preservation of cell proliferation capacity and, consequently, the maintenance of tissue regenerative potential at a high level during the remote postoperative period, up to six weeks.

Conclusion. This research showcases the efficacy of employing a combination of autografts and xenografts, a conclusion drawn from comprehensive histological and immunohistochemical analyses. Consequently, this approach presents a promising and innovative alternative to conventional methods for soft tissue volume and esthetics reconstruction.

Relevance. The oral microbiota, including its interactions with viruses, plays a crucial role in an individual's immune system. Investigating these microbiota-virus associations as biomarkers for personalized health assessments through advanced laboratory testing is an emerging research field.

Materials and methods. This study conducted an extensive clinical laboratory examination of the oral microbiota-virus associations across various biotopes of the digestive tract in 417 participants across different health and age categories: namely, generally healthy children and adolescents in health groups I and II (n = 253, with 456 microbiota-virus associations from 127 individuals); health group III (n = 229, with 436 microbiota-virus associations from 225 individuals); and preterm infants classified by degree of low birth weight (n = 271, with 398 microbiota-virus associations from 55 individuals).

Relevance. The oral microbiota, including its interactions with viruses, plays a crucial role in an individual's immune system. Investigating these microbiota-virus associations as biomarkers for personalized health assessments through advanced laboratory testing is an emerging research field.

Materials and methods. This study conducted an extensive clinical laboratory examination of the oral microbiota virus associations across various biotopes of the digestive tract in 417 participants across different health and age categories: namely, generally healthy children and adolescents in health groups I and II (n = 253, with 456 microbiotavirus associations from 127 individuals); health group III (n = 229, with 436 microbiota-virus associations from 225 individuals); and preterm infants classified by degree of low birth weight (n = 271, with 398 microbiota-virus associations from 55 individuals).

Results. Our findings showed a decrease in indigenous microbiota by 9.7% and an increase in potentially pathogenic microbiota by 19.8% across age groups (p ≤ 0.05). There was also a 5.8-fold increase in the viral components, notably Epstein-Barr virus and HHV-6, in the oral cavities of generally healthy children in health groups I and II. In health group III children, there was a notable increase in opportunistic pathogens and the detection of cytomegalovirus DNA (38.1%, p ≤ 0.05). Furthermore, the predominance of Staphylococcus species in the opportunistic pathogenic microbiota, which correlates with low birth weight, was identified as a prognostic indicator of poor microecological conditions and decreased immune resistance in preterm infants undergoing prolonged hospital care, with detection rates of 19% in extremely low birth weight and 4% in low birth weight groups (p ≤ 0.05).

Conclusion. Evaluating immune resistance in children of various health statuses and age groups through the analysis of oral microbiota-virus associations is advisable for outpatient healthcare settings. This assessment can guide the provision of comprehensive physical exams for children and adolescents and the formulation of personalized treatment plans based on identified risk groups.

Relevance. The transgingival part of the prosthetic components of implant systems is a substrate for the primary adaptation of soft tissues and the formation of the gingiva collar a complex of tissues acting as a biological barrier and ensuring the normal functioning of artificial supports in the microenvironment of the oral cavity. At the same time, the morphology of the surface of the abutment which will be in constant contact with the gingiva part during the loading period is of great importance. A change in the properties of the surface morphology of the transgingival part of orthopedic structures is fraught with the formation of a microbial bio-film in the subgingival zone with the with the subsequent development of mucositis and peri-implantitis. Based on the above, the study of the surface characteris tics of orthopedic components of implantation systems seems relevant in the light of understanding the pathogenesis of inflammatory processes and assessing risk factors for the development of complications of dental implantation.

Purpose. Study of differences in the elemental composition and features (or characteristics) of the surface morphology of orthopedic components transgingival implant abutments from a number of modern manufacturers.

Materials and methods. Prosthetic components from five manufacturing companies were selected for the study. For the assessment, elemental analysis of the surface of the components was used using a Hitachi S-3400N scanning electron microscope with an Oxford Instruments X-Max20 energy dispersive spectrometer, as well as morphological analysis using a TESCAN VEGA 3 scanning electron microscope. The samples were assessed for surface uniformity, the presence of inclusions, cracks and marks.

Results. The study revealed significant differences in the components both in elemental composition and surface topography. The results obtained can be summarized in the form of a table.

Conclusion. The method of comprehensive surface assessment used in the study can be recommended for describing and comparing orthopedic components from different manufacturing companies.

Relevance. Periodontal diseases are among the most prevalent conditions affecting the oral cavity, often leading to tooth loss. Comorbid conditions, especially type 2 diabetes mellitus (DM2), can significantly influence the health of periodontal tissues by altering microcirculatory dynamics.

Materials and methods. This study aimed to evaluate periodontal tissue health in patients with chronic periodon titis, both with and without DM2 comorbidity. Periodontal microcirculation was examined using the Laser Doppler Flowmetry (LDF) technique, specifically with a LAKK-M device (Russia). A total of 106 patients were recruited and categorized into four groups based on their DM2 status and the severity of chronic periodontitis.

Results. The study revealed group-specific differences in periodontal microcirculation parameters, analyzed through nonlinear dynamics. Notably, patients with chronic periodontitis and comorbid DM2 exhibited the most significant alterations. A phase portrait analysis further delineated the microcirculation health disparities between groups.

Conclusion. There were marked differences in microcirculation health among chronic periodontitis patients with and without DM2 comorbidity, as evidenced by significant changes in the nonlinear dynamics-based phase portraits.

Relevance. In approximately 50.82% of instances, sialadenosis of the parotid salivary gland (PSG) emerges as a sequel to gastrointestinal (GI) disorders. This condition is characterized by diminished saliva production (hyposalivation) and enlargement (hypertrophy) of the gland. In Russia, the prevalence of GI diseases linked to acid-related issues and H. pylori infection (HP) is notably high. The therapeutic approach to this pathology focuses on the eradication of HP, employing various treatment strategies that incorporate the use of a proton pump inhibitor (PPI). The efficacy of PPIs hinges on their ability to inhibit the proton pump, which is situated in the endothelial lining of the smooth muscle vessels, including those that encase the acinar cells of the PSG. An essential aspect of parotid secretion is its protective role, which is executed through the production of secretory immunoglobulin A (sIgA) within the gland's ductal system. Therefore, exploring the clinical progression of PSG sialadenosis under eradication therapy regimens holds substantial significance.

Materials and methods. Between 2021 and 2023, 80 patients with parotid salivary gland (PSG) sialadenosis and Helicobacter pylori stomach infection were studied. They were divided into treatment groups: Group I (25 patients, aged 45.81 ± 6.39 years) received quadruple therapy (standard-dose PPI, amoxicillin 1,000 mg, clarithromycin 500 mg twice daily, and bismuthate tripotassium dicitrate 120 mg four times daily); Group II (55 patients, aged 47.79 ± 7.15 years) underwent standard triple therapy (double-dose PPI, amoxicillin 1,000 mg, and clarithromycin 500 mg twice daily). A control group consisted of 20 nearly healthy individuals (average age 26.46 ± 4.12 years). All subjects underwent comprehensive dental and PSG assessments, including questionnaires, sialometry, and measurement of (sIgA) levels in parotid secretion.

Results. After eradication therapy, Group I patients treated with quadruple therapy exhibited a 1.2-fold reduction in PSG secretory function (p = 0.048) and a 1.2-fold decrease in sIgA concentration (p = 0.035) compared to initial levels. Group II patients receiving triple therapy demonstrated a significant 3.6-fold decline in PSG sialometry parameters (p = 0.002) and a 1.8-fold reduction in sIgA levels (p = 0.006) by the 14th day post-eradication, relative to pre-treatment figures.

 Conclusion. Therefore, patients with GI acid-related conditions linked to HP infection should be evaluated by a dentist for potential PSG sialadenosis. The presence of PSG sialadenosis in individuals with HP stomach infection warrants consideration in the selection and administration of eradication therapy.

Relevance. The increasing prevalence of plaque-induced gingivitis among the working-age population worldwide is leading to more cases of tooth loss and diminished quality of life, highlighting the need for early intervention in periodontal inflammation. Given the rising resistance of periodontal flora to pharmacological treatments and the potential for macroorganism sensitization, exploring the efficacy of non-pharmacological interventions that stimulate adaptive mechanisms in periodontal tissues becomes crucial for managing plaque-induced gingivitis effectively.

Materials and Methods. This investigation details the findings from young adults who consented to participate after being informed about the study's protocol. The focus was on evaluating the clinical and functional health of intact periodontal tissues showing early signs of inflammation at the beginning of the treatment, upon completion, and one month after treatment. In addition to standard periodontal treatment, subjects in the experimental cohort underwent daily vacuum laser therapy sessions using a 650 nm wavelength red laser at a pulse rate of up to 10 Hz. Functional monitoring was performed utilizing vital computer capillaroscopy and dynamic vacuum sampling to evaluate capillary resistance in the microcirculation of periodontal tissues.

Results. Vacuum laser therapy demonstrated significant therapeutic benefits in treating plaque-induced gingivitis, notably reducing the diameter of capillaries by approximately 14.5%. This reduction was attributed to enhanced capillary resistance and normalized microvascular wall permeability. Notably, the time to petechial formation doubled following dosed vacuum application to periodontal tissues, indicating enhanced reticuloendothelial system activity and capillary network regeneration. Patient assessments using the oral health index showed notable improvements in oral hygiene and the absence of inflammation signs.

Conclusion. The study's results affirm the effectiveness of combining vacuum laser therapy with conventional treatment modalities for plaque-induced gingivitis, highlighting its benefits in improving periodontal health without relying on pharmacological interventions.

Relevance. Periodontal inflammations result from chronic, persistent infections triggered by dysbiosis-induced shifts within the microbial community. The high prevalence, multifactorial nature and challenging management of periodontal disease create an environment ripe for ongoing advancements in treatment modalities.

Study objective: To validate, using clinical and laboratory test data, the efficacy of combined treatment involving bacteriophages and probiotics in managing chronic periodontitis.

Materials and methods. A total of 100 patients aged 18 to 45 with periodontal inflammation underwent clinical laboratory examinations before receiving combination treatment. The patients were divided into two groups: Group 1 received conventional treatment based on clinical recommendations, while Group 2 received sequential treatment with a bacteriophage cocktail (targeting 81 phages against A. actinomycetemcomitans, B. licheniformis, B. fragilis, E. cloacae, E. faecalis, K. pneumonia, S. aureus, S. pyogenes, Wolinella spp. among others) followed by a probiotic containing S. Salivarius. Periodontal pocket lavages served as study material. Periodontal pathogens were isolated using real-time PCR, and proinflammatory cytokines were identified via enzyme immunoassay. Statistical analysis was conducted using STATA v.12 software.

Results. Following bacteriophage/probiotic combination treatment, positive changes in study indices and a reduction in periodontopathogenic microbiota were observed. Significant decreases were noted in the levels of key periodontal pathogens: P. gingivalis (5.2-fold decrease, p < 0.001), P. intermedia (3.6-fold decrease, p < 0.001; T. forsythia (3.8-fold decrease, p = 0.905), T. denticola 4-fold decrease, p < 0.001. Levels of IL1-β decreased by factors of 4.29 (p < 0.001) and 8.59 (p = 0.02) in severity subgroups, while IL6 levels decreased by factors of 4.39 (p < 0.001) and 5.94 (p = 0.0002). The lowest TNF-α levels (2.13 in mild CGP subgroup and 2.11 in moderate CGP subgroup) were observed post-treatment (p < 0.001; p = 0.0005).

Conclusion. The combination treatment involving the selected drugs exhibited notable therapeutic effectiveness in the management of chronic periodontitis. This approach not only avoided the necessity for systemic antibacterial agents but also facilitated prolonged remission by eradicating periodontopathogenic microbiota in patients with chronic periodontitis. The etiopathogenetic nature of this treatment represents a promising paradigm in the management of periodontal inflammation.

Relevance. The presence of an orthodontic appliance within the oral cavity enhances the formation of supplementary retention sites for the adhesion of microorganisms. The porosity of acrylic base materials, coupled with their limited polishability, further predisposes the surface to contamination by a biofilm of microorganisms. Evaluating the capacity of materials to attract bacterial adherence to their surfaces represents a crucial phase in patient treatment.

Purpose. This study aims to perform a comprehensive clinical and laboratory investigation into the adhesive properties of orthodontic plate appliances crafted from various dental plastics concerning the mixed oral microbiota.

Materials and methods. In vitro research was conducted, focusing on both removable and non-removable orthodon tic plate appliances featuring a base composed of dental plastics (referred to as Sample 1 and Sample 2). The study included 80 orthodontic patients, comprising 50 children aged 8 to 12 years with removable appliances and 30 children aged 8 to 10 years with non-removable plate appliances. Patients were categorized into two groups based on the material of their appliances: the first group, pertaining to those with removable appliances (Subgroup 1A – Sample 1, 25 individuals, and Subgroup 1B – Sample 2, 25 individuals); the second group, comprising individuals with nonremovable appliances (Subgroup 2A – Sample 1, 15 individuals, and Subgroup 2B – Sample 2, 15 individuals).

Results. In the in vitro investigations, it was observed that the average colonization of removable plates was 100 times lower (4.5 log CFU/ml) compared to non-removable plates (5.5 log CFU/ml; p < 0.05). The average colonization of plates composed of plastic (Sample 2) demonstrated a significant reduction, approximately 200 times (4.0 log CFU/ml), compared to plates made of plastic (Sample 1) (6.0 log CFU/ml; p < 0.05). Upon evaluating the overall colonization of the oral cavity, bacterial growth ranged between 10^6-7 CFU/ml in patients with appliances made of plastic Sample 1, whereas in patients with appliances made of plastic Sample 2, the range was limited to 10^3-4 CFU/ml (p < 0,05).

Conclusion. The statistical analysis reveals a significant disparity in the adhesive activity of microorganisms to orthodontic plate appliances constructed from materials of distinct production origins, with notably lower adherence observed on smoother surfaces (specifically those made of plastic Sample 2). This underscores the importance of meticulous consideration for the polishability properties inherent in the materials utilized in the manufacturing of orthodontic appliances.

Relevance. The oral health of the global population is fundamentally influenced by the incidence and severity of dental caries and periodontal diseases. To combat these issues, developed nations devise and execute preventive programs, tailoring their efforts to address the DEF (Decayed, Extracted, and Filled teeth) index across various age demographics. This approach signifies a paradigm shift, with governments now prioritizing the support of oral health through the introduction of comprehensive preventive strategies aimed at combating prevalent oral diseases. The efficacy of these programs, however, hinges on the occurrence of dental caries and, by extension, the availability of oral health professionals. This availability, in turn, shapes the landscape of health facilities—whether public or private—determining their ability to cater to community needs for oral health services. Consequently, the capacity to implement preventive measures is gauged against these dynamics. Furthermore, the nature and scope of preventive actions within these programs vary from one country to another, influenced primarily by the allocation of healthcare budgets by respective governments.

Materials and methods. Analysis of scientific articles and original research from the PubMed, ResearchGate, ScienceDirect for the last 10 years. The search was carried out by keywords: dental prevention programs in the world, prevention of dental health, organization of preventive care in the world. An examination of research articles and original papers was conducted, drawing from databases such as PubMed, ResearchGate, and ScienceDirect over the last decade. The search employed specific key phrases, including "dental prevention programs worldwide", "dental health prevention", and "global organization of preventive dental care".

Results. The initial advancement towards the creation and implementation of fluoride prophylaxis programs occurred following the discovery of fluoride's presence in water. A pivotal moment occurred in 1949 at Indiana State University when J.C. Muhler introduced the idea of fluoride toothpaste (specifically sodium fluoride) as a means to combat dental caries in children. Subsequent innovations included the use of silver fluoride for argentation. European countries took a holistic approach, emphasizing oral hygiene, fluoride toothpaste usage, and balanced nutrition. Meanwhile, the US and EU shifted towards a preventive approach within health insurance frameworks. A critical factor influencing the effectiveness of these preventive care programs is the availability of oral health professionals per 10,000 people in the population, alongside social workers advocating for healthy living and oral health. The engagement of more oral health professionals in preventive efforts correlates with a reduction in the incidence and severity of common oral diseases.

Conclusion. The six stages of preventive care development and transformation highlight the evolving trends in global dentistry, which are crucial for ensuring the quality of oral health care across different countries. The effectiveness of these programs in individual nations is contingent upon the specific preventive care.

Relevance. The high prevalence of inflammatory periodontal diseases (IPD) and periodontitis, in particular, is a relevant issue since the latter progression leads to alveolar bone destruction and loss of periodontal attachment and is considered one of the principal causes of tooth loss in the adult population. The adverse impact of periodontal pathogens doesn't only extend to the oral organs and tissues, but it also affects general health. There is evidence of a possible relationship between IPD and cardiovascular diseases (CVD). Periodontal pathogens are found in the myocardium, pericardial fluid, heart valves and vascular intima.

Purpose. The study aimed to systematically review the impact of periodontitis on the risk of cardiovascular disease development.

Material and methods. The study systematically reviewed scientific articles and original studies from international and national databases using the PRISMA (The Preferred Reporting Items for Systematic Reviews and Meta-Analyses) checklist for systematic reviews and meta-analyses.

Results. The study detected 1857 articles according to the inclusion criteria. After the duplicate removal, the number of articles decreased to 685. Of these, 137 articles were selected for screening, which contained data from randomized controlled clinical trials, longitudinal studies, cohort studies and case-control studies. Ninety-eight articles were excluded for the lack of clinical diagnoses or clear diagnostic criteria for the studies and the lack of sample representativeness. The experts assessed 39 articles, of which seven were removed due to a high risk of bias. Finally, 32 studies were included in the systematic review.

Conclusion. Thus, the review confirms the hypothesis that there is a relationship between IPD and CVD, such as ACVD, myocardial infarction, peripheral vascular disease, and hypertension. The theory of the pathogenic influence of systemic inflammation caused by the spread of periodontal pathogens in the bloodstream is the key option for grounding this relationship.